产品货号：
KD1604
中文名称：
Flag-Tag mouse monoclonal antibody
英文名称：
Flag-Tag mouse monoclonal antibody
产品规格：
100μL
发货周期：
1～3天
产品价格：
询价
保存：-20℃
The DYKDDDDK peptide is a small component of an epitope which does not appear to interfere with the
bioactivity or the biodistribution of the recombinant protein.It has been used extensively as a general epitope tag in expression vectors.As a member of Tag antibodies,Flag tag antibody is the best quality antibody against
DYKDDDDK in the research.As a high-affinity antibody,Flag tag antibody can recognize C-terminal,internal,
and N-terminal Flag-tagged proteins.
Product Name:Flag-tag mouse monoclonal antibody
Clone Number:3C6
Isotype:IgG1
Immunogen:A synthetic peptide DYKDDDDK coupled to KLH.
Tested applications and dilutions:WB 1:2000～5000 IP 1:200 IF 1:1000
Produce anti-FLAG antibody affinity column purify FLAG recombinant proteins
Form:Liquid
Concentration:1mg/ml in PBS,pH7.4,containing 0.02% sodium azide and 50% glycerol
Storage:Flag antibody is stable for 1 month at 2～8℃.For long term storage,Aliquot and store at -20℃ or below.
Avoid repeated freezing and thawing cycles.
A:HEK 293 cell lysate without transfected
B:HEK 293 cell lysate transfected with FLAG-tag protein
C:IP (PBS + anti- FLAG mAb 4℃ overnight)
D:IP (HEK293 transfected+protein G+anti- FLAG mAb 4℃ overnight)
E:IP (HEK293 transfected+RIPA+Protein G 4℃ overnight)
F:Multi Tag Recombinant protein (expressed in E.coli)
TP=Target Protein HC=Heavy chain LC=Light Chain
IF analysis of 293T cells transfected with a FLAG-tagged protein,
using EASYBIO FLAG-Tag (3C6) Mouse mAb at a 1:2000 dilution
(blue DAPI,red anti-FLAG)
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